| Main idea | Animal model | Cell model | Approach | Ref. |
| In vivo: ↑ trabecular bone mass ↓ decreased osteoclast number and serum NTx In vitro: ↓ osteoclast differentiation ↑ expression of alkaline phosphatase and mineralization in MC3T3-E1 cells | C57BL/6J mice treated with adenovirus expressing lacZ or adiponectin | Treat MC3T3-E1, CD14+ cells, bone marrow macrophage with adiponectin | Micro-CT, EIA (NTx), Histological examination (TRAP+), real-time (ALP), Alizarin Red S staining | |
| In vivo: No difference between overexpressing adiponectin and WT In vitro: 1. ↓ osteogenesis in Ad−/− mice bone marrow cell 2. Treat adiponectin recombinant protein inhibit osteogenesis | Ad−/− mice, Ad-Tg mice | Bone marrow cell (from adult mouse long bones, neonatal calvarial osteoblasts, osteoclast precursor M-CSF- dependent bone marrow macrophage, ST2 cells | Histtological analysis, osteoclast formation assay, immunoprecipitation and immunoblotting | |
| In vivo: ↑ Trabecular bone volume and trabecular number in 14 week Ad KO mice. In vitro: ↓ osteoclastogenesis in rat and human osteoclast | AdKO mice (C57BL/6J) | Rat and human primary osteoblast, osteoclast | Micro-CT, primary osteoblast culture | |
| In vivo: ↓ osteoclastogenesis and bone resorption via APPL1-mediated suppression of AKT1 In vitro: ↓ osteoclast differentiation by RANKL in RAW264.7 and decreased expression of osteoclastogenic regulator (NFAT2, TRAF6) | ADN-KO mice | RAW264.7 | Histological examination, micro CT |